To understand the current status of Chiroptera species diversity in the northwest and southwest regions of China, we conducted a field survey in Qinghai in July 2023. Six bat specimens (3♀ 3♂) were collected using harp nets and mist nets in Xunhua Salar Autonomous County, Haidong City, Qinghai Province, China. Morphological and molecular systematics methods are used for species identification. These bats possesses a medium body size, with a forearm length of 51.45 ± 2.18 mm (49.03﹣54.29 mm, n = 6). The ear measures 16.96 ± 1.52 mm (15.30﹣18.86 mm, n = 6) and does not extend beyond the rostrum tip when folded forward. The tragus is short at 7.48 ± 1.07 mm (5.83﹣8.65 mm, n = 6), approximately half the ear length. The skull snout is low and upturned with a low and flat forehead. The greatest skull length measures 17.99 ± 0.13 mm (17.85﹣18.17 mm, n = 4), with a braincase height of 7.00 ± 0.27 mm (6.60﹣7.22 mm, n = 4). Upper gear row length 6.84 ± 0.25 mm (6.52﹣7.12 mm, n = 4) (Appendix 1)The second upper and lower premolars are extremely small and located in the dentition. The dorsal pelage exhibits brown coloration with blackish-gray tips, while the ventral pelage displays black hairs tipped with grayish-white.(Fig 1) These characteristics demonstrate congruence with the external morphology and cranial features of Myotis pequinius. The Bayesian phylogenetic analysis results showed that the Qinghai sample was clustered with the M. pequinius from other regions. The genetic distance between the Qinghai specimen and theM. pequinius distributed in Shaanxi is the closest(Appendix 2). The species were identified as M. pequinius, a new record in Qinghai Province. The research results enriched the species diversity and distribution data of the Chiroptera in China, and providing basic information for the phylogenetic geography research of the M. pequinius.