Three snake specimens were collected in Pu’an County, Guizhou Province in August 2020. The specimens resemble Pareas niger based on morphological comparisons. The phylogenetic trees constructed based on the mitochondrial cytochrome b (Cyt b) gene sequences show that the newly collected specimens are clustered closely with a topotype of P. niger (Kunming, Yunnan) (Fig. 2), and the genetic distance between them is 0.4%, which is much smaller than interspecific genetic distance of Pareas (5.60%﹣23.90%). According to morphological comparisons and molecular phylogenetic results, the newly collected specimens should be P. niger, which is a new record of reptiles in Guizhou Province.