On October 17th 2020, one female bat was collected by handheld-netting from Zhuoni Country, Gansu Province (103°30′37″ E，34°35′00″ N). Its forearm length is 59.98 mm; ears are large and connect at the anterior bases; tragus fringed with hair anteriorly and dorsally; upper lip is wrinkled; muzzle projects beyond upper lip; terminal half tail extended beyond the membrane; feet are of a distinct fleshy pad; toe edges with white bristles; dorsal fur is black-gray and paler at base. The skull is elongated with a greatest length of skull (GTL) of 24.05 mm; zygomatic arch is straight; its upper incisors and upper canines are large, while lower incisors are small. Its dental formula is 1.1.2.3/3.1.2.3 = 32. All characteristics mentioned above are consistent with the East Asian Free-tail Bat (Tadarida insignis). Such identification is also supported by phylogenetic evidence based on Cytochrome b gene segment (Cyt b). The finding represents the first chiropteran record of T. insignis in Gansu Province, China.