Three tailless amphibian specimens were collected in Puhe Wetland Park (41°41′54″ N, 122°52′19″ E, elevation 35 m) in Shenyang in September 2021 (Fig. 1, with Fig. 3 showing the habitat). In this study, the morphological characteristics of the collected specimens were compared and analyzed in detail with P. chosenicus and P. plancyi. The 12S rRNA and 16S rRNA gene of 3 samples were amplified and sequenced by second-generation high-throughput sequencing. The 12S rRNA and 16S rRNA gene information of 19 species of Pelophylax distributed in China and abroad (including a species of Rana as an outgroup) were obtained in NCBI GenBank database and existing reports. Phyml 3.0 to construct maximum likelihood phylogenetic tree. Morphological analysis shows that there are interspecific differences between our specimens and P. plancyi, which is consistent with the type origin species of P. chosenicus. The results of ML phylogenetic tree show that the Pelophylax specimens collected in Shenyang Puhe Wetland Park and the type origin (Korea) specimens of P. chosenicus gather into one branch (Fig. 2), with a high support rate (95%). By comprehensive comparison with morphological and phylogenetic analysis, the specimens we collected should be the P. chosenicus of the genus Pelophylax in the family Anura, and be a new record of amphibian distribution in China.