Abstract:The breeding ecology of Rana hanluica endemic in China remains understudied. We therefore monitored the morphological traits, breeding habitat, and reproductive behavior of R. hanluica during the breeding season from October 2022 to May 2023 in Ganjiao Village, Leigongshan National Nature Reserve, Guizhou Province. The results indicated no significant morphological dimorphism between sexes, a male-biased sex ratio during the breeding season, and rice paddies as the primary breeding habitats. Oviposition was first observed on October 10 and last observed on October 30. Eggs were deposited in paddy fields, with a clutch size of 927.4 ± 62.6 eggs (n = 15), an egg diameter of 2.81 ± 0.16 mm (n = 7), a hatching period of 12.46 ± 1.36 d (n = 15), a hatching rate of 73.5% ± 6.67% (n = 3), and a tadpole total length at hatching of 6.23 ± 0.44 mm (n = 5). It takes approximately 220 d from fertilization to the end of metamorphosis.