In August 2023, a chiropteran diversity survey was conducted in Xizang Autonomous Region, China. During the survey in Medog County (95°20′11″ E, 29°19'16″ N; elevation:1 107 m), we collected two male bat specimens (2 ♂) by using harp traps and measured their morphological and skull characteristics with electronic digital calipers. The cytochrome b (Cyt b) gene sequences of the two bat specimens (specimen numbers: XZ-2023-044 and XZ-2023-045) were sequenced and uploaded to the NCBI database (Table 2). A phylogenetic tree was constructed with the maximum likelihood method in IQ-TREE, and the Kimura 2-parameter model was adopted to calculate the intraspecific, interspecific, and intergeneric genetic distances of Harpiola isodon based on Cyt b gene sequences. Morphological measurements of the two specimens were head-body lengths of 35.72 mm and 36.56 mm, forearm lengths of 33.58 mm and 33.68 mm, and tibia lengths of 14.14 mm and 14.51 mm (Table 3). The specimens exhibited small, rounded auricles with slightly curved, slender tragus tips. Dorsal pelage was predominantly yellowish-brown, with black hair bases and golden tips; ventral pelage was pale yellow with black bases and pale yellow tips (Fig. 1). Skull lengths were 17.06 mm and 16.75 mm, with a generally rounded braincase (Fig. 2). The frontal bone displayed a gradual elevation from the anterior to the posterior, with a slight central depression. The sagittal crest was absent, whereas a well-defined lambdoid crest was present. Zygomatic breadth exceeded braincase width (Fig. 2). The upper first incisors were absent. The upper incisors are about two-thirds that of the C¹ in height, and C₁ and premolar teeth are similar in both bulk and height, among which C₁ is slightly less in height, and P₂ is smaller in basal area (Fig. 2). The dental formula was 2.1.2.3/3.1.2.3 = 34. According to the aforementioned morphological characteristics, the two bat specimens were taxonomically identified as H. isodon. Phylogenetic analysis based on Cyt b gene sequences demonstrated that the collected specimens formed a monophyletic clade (bootstrap support of 100) with reference sequences of H. isodon from Taiwan (the type locality; GenBank accession numbers: GQ168914 and GQ168920) and Yunnan (PP476123 to PP476127). Genetic distance analysis revealed minimal intraspecific divergence (< 5.0%), while the interspecific divergence ranged from 14.7% to 18.6% compared with closely related genera. The molecular data provide robust support for the taxonomic assignment of this specimen as H. isodon. The congruence between morphological characteristics and molecular phylogenetic results conclusively confirms the species identification. This discovery significantly extends the known geographic distribution of H. isodon to western China.