[Objectives] Chigger mites are the exclusive vector of scrub typhus (tsutsugamushi disease). Nujiang Lisu Autonomous Prefecture is located in the northwest of Yunnan Province, where scrub typhus has been prevailing. Therefore, the investigation and analysis of chiggers in Nujiang Prefecture is of great significance for the prevention and control of scrub typhus in this region. [Methods] The present study was conducted in Nujiang Prefecture from 2002 to 2022, which involves the field investigation, collection and specimen making of chigger mites, taxonomic identification of chigger mites, analysis of sample completeness, analysis of species composition of chigger mites, and visualization of host-chigger mite relationship. The sample completeness was determined through the curve of species sparsity, extrapolation and coverage index.The infestation indexes of chiggers were routinely calculated. Infestation indices include prevalence (P_M, %), mean abundance (A) and mean intensity (I). Spearman correlation coefficient was used to analyze the interspecific relationships among some main chigger species. The bipartite network analysis was used to analyze the mutual relationship between host species and chigger species. [Results] The results showed that the coverage index reached 99.55% (Fig. 2). A total of 2 036 rodents and other sympatric small mammal hosts were identified as 4 orders, 9 families, 26 genera and 44 species. From the body surface of 2 036 hosts, 3 087 chigger mites were collected and taxonomically identified as two families, three subfamilies, 12 genera and 66 species. The 66 chigger species were listed in the form of taxonomic checklists (Table 1). The overall infestation prevalence, mean abundance and mean intensity of chigger mites on their hosts were P_M= 7.02%, A = 1.52 and I = 21.59, respectively. The taxonomic tree diagram at the level of families and genera showed that the number of the genus Leptotrombidium were abundant with the constituent ratio C_r= 26.47% (817/3 087) (Fig. 1). Among the 66 identified species, there were two dominant species (C_r= 56.24%, 1 736/3 087), one vector and five potential vector species of scrub typhus. Of the six vector and potential vector species of chigger mites, the vector L. scutellare and potential vector L. rusticum were the major species with the highest number of individuals (Table 2). The bipartite network analysis between chigger mites and their hosts showed that a single chigger species can parasitize a few host species, and a single host species can harbour a few chigger species with low host specificity (Fig. 3). A positive correlation existed among some chigger mite species in host selection (Fig. 4). [Conclusion] The sampling of field investigation is sufficient for this study, and the species diversity of chigger mites is high in Nujiang Prefecture. The low host specificity of chigger mites, the abundance of the genus Leptotrombidium and the coexistence of six vector or potential vector chigger species in Nujiang Prefecture would increase the potential risk of transmission and focus persistence of scrub typhus in the region.