Wild birds play a significant role in the maintenance and transmission of various zoonotic pathogens. Among them, the Oriental Magpie Pica serica is considered a representative terrestrial wild bird species. Elucidating its role in the transmission of zoonotic pathogens is of particular importance. In this study, deceased Oriental Magpies were collected from the campus of Capital Normal University, and comprehensive morphological and molecular analyses were performed on their intestinal parasitic nematodes. First, necropsies of deceased Oriental Magpies revealed a significant number of cylindrical parasites, measuring 4 to 15 cm in length, in their intestines. A stereoscopic zoom microscope (SZM) and a scanning electron microscope (SEM) were subsequently used to examine the morphological characteristics. Genomic DNA was then extracted from Ascaridia galli, and the cytochrome oxidase subunit I (COX1) gene was amplified via polymerase chain reaction (PCR). Positive clones were sequenced to identify target DNA inserts. The resulting sequences were assembled manually via BioEdit (v7.0.9) and aligned with A. galli reference sequences from GenBank by BLAST. A phylogenetic tree was constructed via the Neighbor-Joining (NJ) method with 1 000 bootstrap replicates in MEGA 9, along with a barcoding gap assessment. The parasite exhibited a three-lipped anterior oral aperture, a visible anus at the tail end, and a vulva anterior to the anus, which suggested that this specimen was female A. galli (Fig. 1). Subsequent phylogenetic analysis indicated that the parasite sequence obtained clustered with reference sequences of A. galli from chicken hosts (Fig. 2), with sequence similarity greater than 90%, conclusively identifying the parasite as A. galli. This study identified A. galli in Oriental Magpies inhabiting urban natural environments, thereby expanding current knowledge of the parasite’s host range and highlighting the important role of wild urban-dwelling birds in public health surveillance.