During a field survey of amphibians in Meihua Mountain National Nature Reserve, Longyan City, Fujian Province in October 2024, four specimens (1 male and three females) of Rana were collected. The study aims to accurately identify the species of these specimens through morphological and phylogenetic comparisons, enriching the diversity of amphibians in Fujian Province. Morphological identification was performed by comparing the diagnostic features described in the database of Amphibia China (http://www.amphibiachina.org/), as well as by comparing the measurements of 23 morphological indicators. Meanwhile, molecular phylogenetic identification was performed based on mitochondrial 16S ribosomal RNA (16S rRNA) gene fragments. The 16S rRNA gene fragments were amplified with primer pairs of L3975: 5′-CGC CTG TTT ACC AAA AAC AT-3′ and H4551: 5′-CCG GTC TGA ACT CAG ATC ACG T-3′. Corresponding sequences of 37 species of Rana were downloaded from GenBank (Table 1) and R. luteiventris and R. muscosa were selected as outgroups. DNA sequences were aligned and trimmed by Clustal W and the nucleotide substitution model GTR + I + G was selected based on the Bayesian information criterion (BIC) in MEGA 11. RaxmlGUI 2.0 and MrBayes 3.2.4 were used to build maximum likelihood (ML) and Bayesian inference (BI) phylogenetic trees, respectively. The uncorrected genetic distances within Rana were calculated via MEGA 11. The measurements of 23 morphological indicators were shown in Table 2, which indicated that snout-vent length of the collected specimens ranged from 59.74 to 63.04 mm; the head length was longer than the head width, and the snout was blunt pointed, obviously extending beyond the lower lip; the lengths of lower arm and hand were less than half of the body length; the hindlimbs were long, and the tibial tarsal joint extended anteriorly beyond the snout. The characteristics of skin and coloration: dorsal skin smooth, with scattered brown maculations in females; ventral skin smooth, light yellow, throat with scattered red punctate speckles and the posterior femur and its ventral surface with flat tubercles; dorsal and lateral surface olive-yellow in breeding males and reddish brown in breeding females; the dorsal skin on both hindlimbs with 9 or 10 neat and narrow black-brown horizontal stripes, without male lines (Figs. 1, 2). The morphological characteristics are consistent with the diagnosis characteristics of R. hanluica. Additionally, our specimens are larger than the topotype and those collected from other localities (Table 3). The new sequences of our specimens were deposited in GenBank with the accession number PQ608565﹣PQ608568. The BI and ML phylogenetic trees based on 16S rRNA gene fragments had identical topologies, which showed that our specimens and R. hanluica from Guangxi, Chongqing, and Hunan formed a monophyletic clade with the posterior probability of 0.99 and bootstrap support value of 100% (Fig. 3). The uncorrected p-distances among them were zero, which were much smaller than the minimum genetic distance (2.20%) between these samples and other Rana species. Based on the comprehensive comparisons of morphology and phylogenetics, the collected specimens were identified as R. hanluica, a newly record species of amphibian in Fujian Province. This discovery further enriches the geographical distribution information of R. hanluica and the diversity of amphibians in Fujian Province.