Rana sangzhiensis was initially described by Shen Yuhui in 1986 from Hunan, China. On August 27, 2024, a female subadult specimen (GXNU090506) was collected from Rongshui Miao Autonomous County, Liuzhou, Guangxi. Subsequently, on October 15, 2024, a male adult specimen (GXNU102338) was collected from the same location. Morphological comparison revealed similarities to R. sangzhiensis (Figs. 1, 2). In addition, the 16S ribosomal RNA (16S rRNA) gene sequences were analyzed, with sequencing peak maps evaluated and manually corrected. Additionally, 60 sequences from 18 Rana species were retrieved from GenBank for comparison (Table S1). Phylogenetic analyses were conducted using Bayesian inference (BI) and Maximum likelihood (ML) methods via PhyloSuite. Species boundaries were delineated via the Bayesian Poisson Tree Processes (bPTP). The phylogenetic analysis based on the 16S rRNA gene indicated that the collected specimens clustered with R. sangzhiensis specimens (Figs. 3, 4), exhibiting uncorrected pairwise distances (p-distance) of 0.2% (Table 2). This p-distance was notably lower than the interspecific p-distances observed within the genus Rana (0.8%﹣8.4%) and significantly lower than the p-distance to the outgroup (11.6%). Integrating morphological characteristics with molecular phylogenetic evidence, we conclude that the two specimens collected from Guangxi belong to R. sangzhiensis. This finding represents a new record for amphibian distribution in Guangxi and extends the known distribution range of the R. johnsi group.