A total of 25 Barbastella bats (KT13742, KT13743, KT13753, PY13770, and BX13777﹣BX13797) were captured by our research group in June and July 2013 in Pingliang City of Gansu Province, Guyuan City of Ningxia Hui Autonomous Region, and Xianyang City of Shaanxi Province. They are medium-sized bats with forearm length ranging from 42.00 to 45.58 mm. The hind-foot length is less than half of the tibia length, and the length of the 3rd metacarpal bone to the 5th metacarpal bone decreases in descending order (Table 1). These bats have nearly a square outline of ears with transverse ridges (Fig. 1a). Their ears join across the forehead, with a small lobe on the middle outer edge of each pinna (Fig. 1b). The length of skulls ranges from 14.81 to 15.74 mm (Table 1), while both sagittal and lambdoidal crests are weak (Fig. 1d). The dental formula is 2.1.2.3/3.1.2.3 = 34 (Fig. 1c). With 1 000 bootstrap replicates, phylogenetic trees were reconstructed based on cytochrome b (Cyt b) and NADH dehydrogenase subunit 1 (ND1) gene sequences. The specimens in this study clustered with B. beijingensis, rather than B. darjelingensis in both cladograms of Cyt b (Fig. 2) and ND1 (Fig. 3) genes. By comparing external morphology, skull characteristics, and phylogenetic data with B. beijingensis and B. darjelingensis, these bats are identified as B. beijingensis, which are new records of Chiroptera for Gansu Province, Ningxia Hui Autonomous Region, and Shaanxi Province. The above specimens are preserved in the Institute of Zoology, Guangdong Academy of Sciences.