This study aimed to document and analyze a newly discovered bat specimen of the genus Barbastella in Hebei Province, China and elucidate its taxonomic status through integrated morphological and molecular methodologies. In September 2024, a male Barbastella bat (specimen number: HEB-2024-058) was captured via a mist net in Hezhang Township, Shexian County, Handan City, Hebei Province. Comprehensive external morphological and cranial measurements were recorded, and high-resolution photographs were taken to facilitate detailed morphological comparisons. Muscle tissue was collected for DNA extraction, and the mitochondrial ND1 gene was amplified and sequenced. Phylogenetic analysis was conducted based on the published sequences of B. darjelingensis, B. beijingensis, and B. barbastellus, with Plecotus auritus and P. austriacus serving as outgroups. A maximum likelihood tree was constructed in Mega 11.0 to evaluate phylogenetic relationships. The specimen exhibits a forearm length of 39.9 mm and ears measuring 13.5 mm in length (Appendix 1). The ears are characterized by a nearly square morphology, with their inner edges converging across the forehead (Fig. 1). Notably, the margins of the ears lack lobes. Both the dorsal and ventral surfaces are covered in hairs that display a dark brownish-black coloration with slightly lighter tips. The hind-foot length, at 4.8 mm, is notably short, measuring less than one-third of the tibia length, which is 18.6 mm. Cranially, the specimen presents a greatest skull length of 15.0 mm, with a condylobasal length of 14.7 mm, which surpasses that of B. darjelingensis and B. beijingensis (Fig. 2). However, its upper canine width (3.4 mm) and condylocanine length (12.6 mm) are comparatively smaller. These morphological characteristics closely correspond with those of B. darjelingensis. Phylogenetic analysis of the ND1 gene further corroborated this classification, as the Hebei specimen formed a well-supported clade with B. darjelingensis, distinct from B. beijingensis (Fig. 3). Integrative morphological and molecular analyses have confirmed the specimen as B. darjelingensis, marking the first documented occurrence of this species in Hebei Province. This discovery expands the known geographical distribution of B. darjelingensis within China and highlights the ecological significance of the Taihang Mountains as a viable habitat for this species. Further investigation is necessary to evaluate its population dynamics and conservation needs.