[Objectives] Studying the changes of cell and tissue morphology in the tail regeneration process of Gekko reevesii during winter can help understand the mechanism of tail regeneration in G. reevesii. [Methods] The squeezing method was employed to induce tail self-amputation of G. reevesii, and the tail regeneration trends and influencing factors of G. reevesii at different time points (0 d, 0.5 d, 1 d, 3 d,7 d, 10 d, 15 d, 20 d, 30 d, 45 d, 60 d, and 90 d) after tail self-amputation were analyzed. Then, paraffin and frozen sections were prepared to analyze the characteristics of cells and tissues of the original tail and the regenerating tail in G. reevesii, respectively. The generalized linear mix model was adopted to analyze the effects of temperature on weight changes before and after tail regeneration. [Results] When G. reevesii amputated its tail, the skin on one side of the self-amputation site first cracked, and then the muscles were pulled out. Finally, the tail vertebrae were broken. During days 0﹣3 after tail amputation (Fig. 4), the main characteristics were small blood loss from the tail artery, no tissue contraction, exposure of vertebrae and fat, formation of blood clots, and collapse of the outer skin around the wound site to reduce the wound size. During days 3﹣30 after tail amputation (Fig. 5), the main characteristics were the contraction of exposed soft tissue (e.g. adipose tissue and axial muscle tissue), collapse of the tunica, and greater prominence of the intervertebral remnants due to the lack of muscle tissue. During days 30﹣45 after tail amputation (Fig. 6), the main features were the disappearance of exudate clots and exposure of newly formed wound epithelium, relatively flat self-section, no longer existence of the previously protruding vertebral residue, and not obvious growth. During days 45﹣90 after tail amputation (Fig. 7), although the wound healed, no tail regeneration was observed in most individuals. However, on days 60 and 90, it was found that two individuals formed regenerated tail blastema, with an average regeneration rate of 0.01 mm/d (Fig. 2). Temperature did not affect the weight changes before and after tail amputation (B = 0.001，95% CI ﹣0.006 to 0.004，Wald x2 = 0.130，P = 0.719). [Conclusion] The above results indicate that after tail amputation of G. reevesii in winter, most individuals do not regenerate the tail and only keep wound healing at a low rate.