Shanghai is located in the coastal area of eastern China and lies in the subtropical monsoon climate zone. Its unique geographical location and ecological environment provide suitable conditions for the diversity of reptiles. Therefore, it has long been an important area for biodiversity research. Notwithstanding the existing research on reptiles in Shanghai, the expansion of cities and changes in the ecological environment over the past few decades may have led to a lack of comprehensive investigations and reporting of new species records.On July 12, 2022, a grass lizard (specimen number:DT20220712) was photographed in Dongtan Wetland Park in Chongming District, Shanghai (31°52′48″ N, 121°83′31″ E). This individual had four pairs of chin shields and one pair of inguinal pores, as well as two longitudinal white stripes on its dorsal and lateral body. These morphological features are similar to those of Takydromus wolteri. On October 30, 2023, a male grass lizard specimen (specimen number:DT20231030) was collected from the same location. A digital caliper was used to measure 21 morphological characteristics of the grass lizard with an accuracy of 0.02 mm. Based on the morphological descriptions, the specimen was identified as T. wolteri.IQ-tree software was used to construct a maximum likelihood phylogenetic tree based on mitochondrial ND2 gene fragments. The result showed that our sample clustered robustly with T. wolteri (GenBank accession number:JX181764) with a high support value of 98.84% (Fig. 2). Furthermore, the genetic distance calculated via the p-distance model between our specimen and T. wolteri was only 4.5%, which was significantly lower than the genetic distances (15.2% to 44.0%) observed between Takydromus species (Table 2). Based on both morphological and genetic data, the newly collected specimen was identified as T. wolteri (Reptilia, Lacertilia, Lacertidae). This is a new record for reptile species in Shanghai, further enriching the biodiversity in this region.