Opisthotropis zhaoermii was previously known only from Guzhang County, Hunan, and Xiushan, Chongqing, China. On August 28, 2024, two female snake specimens (GXNU090517 and GXNU090518) were collected from Rongshui Miao Autonomous County, Liuzhou, Guangxi. The morphological characteristics of the two specimens were similar to those of O. zhaoermii (Figs. 1﹣3). The sequencing peak maps of 2 sequences of the mitochondrial cytochrome b (Cyt b) gene were evaluated and manually corrected in this study, and 31 sequences from 16 species of Opisthotropis were retrieved from GenBank (Table 1). PhyloSuite was used for Bayesian inference (BI) analysis and maximum likelihood (ML) analysis. The phylogenetic analysis based on Cyt b revealed that the specimens and O. zhaoermii specimens clustered together (Fig. 4), with uncorrected pairwise distances (p-distance) approaching 0 (Table 3), which were less than the p-distances between species of Opisthotropis (0.048﹣0.187). According to both morphological characteristics and molecular phylogenetic analysis, the two specimens collected in Liuzhou were identified as O. zhaoermii, which was a new record species for reptile distribution in Guangxi Zhuang Autonomous Region, China.