In August 2023, a female Myotis bat was captured by a harp trap in Shangcheng County, Xinyang City, Henan Province. The echolocation calls of the specimen were recorded during its stationary and exploratory behaviors. The external morphology and cranial parameters were measured, and genomic DNA was extracted from the wing membrane tissue. The complete sequence of the ND1 gene was amplified to facilitate the construction of a phylogenetic tree for species identification. Morphometric analysis indicated that the specimen was relatively large, with a forearm length of 64.3 mm. The dorsal fur exhibited a dark gray-brown color with nearly black roots, while the ventral fur was gray (Fig. 1a). The tragus was narrow and elongated, measuring 11.1 mm, approximately half the length of the ear (Fig. 1b). The wing membrane originated at the base of toes, and the tail membrane commenced at the ankle joint (Fig. 1c). The skull was slightly flattened, featuring a prominent sagittal crest (Fig. 2). The echolocation calls were characterized by a frequency-modulated type, comprising two harmonics (Fig. 3), with a peak frequency of 44.1 ± 4.9 kHz. The maximum likelihood phylogenetic tree constructed based on the ND1 gene (Fig. 4) indicated that the Myotis specimen from Henan shared the same clade with M. chinensis (GenBank accession number:AB106567), exhibiting minimal sequence divergence and a genetic distance of 0.003. Conversely, the genetic distances between the Henan specimen and both M. blythii and M. pilosus exceeded 0.120. According to the data from external morphology, cranial measurements, and the phylogenetic analysis, the Myotis specimen from Henan was identified as M. chinensis, marking a novel record of bat species distribution in Henan Province.