[Objectives] Two species of foliar nematodes were extracted from the leaves of Tillandsia fasciculata and the root rhizosphere of Vanda subconcolor, both of which were imported from Thailand, using a modified Baermann funnel technique. The taxonomic status of the two intercepted Aphelenchoides populations was determined through comprehensive morphological and molecular analyses. [Methods] For morphological analyses, nematode specimens were extracted, fixed, dehydrated and permanently mounted in anhydrous glycerin on glass slides. Specimens were photographed, measured and illustrated using a Leica DMi8 microscope. Morphometric measurements were carried out and analyzed in accordance with the de Man formula. For molecular analyses, genomic DNA was extracted from individual fresh nematodes. Two sets of universal primer pairs were employed for PCR amplification targeting both the 18S ribosomal DNA (rDNA) and the D2-D3 expansion segment of 28S rDNA. The resulting PCR products were subjected to separation, purification and sequencing. The newly obtained rDNA sequences were edited using DANMAN software package, compared against the NCBI database via BLAST, and subsequently deposited in the NCBI GenBank database. Phylogenetic analyses were conducted using a suite of bioinformatics tools:PhyloSuite v1.2.3 for extracting related species sequences and identifying the best-fit evolutionary model, MAFFT v7.313 for sequence alignment, MrBayes 3.2.7 for constructing Bayesian inference (BI) phylogenetic trees, and iTOL v6 for tree visualization. [Results] The population densities recovered from T. fasciculata and V. subconcolor were approximately 10 ind per 100 g leaves or roots, respectively. Both populations exhibited morphological similarities to A. besseyi, an economically significant plant-parasitic nematodecharacterized by a star-shaped tail tip and classified within the Aphelenchoides Group 3 as defined by Shahina (1996). Comparative morphological and morphometric analyses revealed that the nematode population from T. fasciculata closely resembled the Fragaria × ananassa-associated populationof A. pseudogoodeyi from Florida, USA, exhibiting overlapping ranges for all morphometric data except the b ratio. Similarly, the nematode population from V. subconcolor demonstrated morphological characteristics consistent with the Medicago sativa-associated population of A. medicagus from the United States, with overlapping measurements for all parameters except the c ratios in females. Phylogenetic analyses based on 18S rDNA and the 28S D2-D3 rDNA sequences further demonstrated the distinct evolutionary relationships among the two Aphelenchoides populations. In the 18S BI tree, the population from T. fasciculata formed a well-supported independent subclade (posterior probability, PP = 1.00) with A. pseudogoodeyi, while in the 28S BI tree, it formed a moderately supported independent subclade (PP = 0.98). Concurrently, the population associated with V. subconcolor clustered robustly with A. medicagusin in both the 18S and 28S BI trees, resulting in well supported independent subclades (PP = 1.00). [Conclusion] Based on an integrated analysis of morphological and molecular evidence, the nematode population derived from T. fasciculata was identified as A. pseudogoodeyi, whereas the population from V.subconcolor was identified as A. medicagus. Both of these nematode species represent new geographical records for Thailand, while T. fasciculata and V.subconcolor also serve as new host record species for the two nematodes, respectively. This study significantly enhances our understanding of the ecological characteristics, geographical distribution, and host range of Aphelenchoides nematodes. Furthermore, it offers a scientific foundation for formulating effective plant protection strategies and phytosanitary measures, aimed at mitigating risks associated with importation of plants from Thailand.