[Objectives] The Irrawaddy River is one of the major rivers in the Indochina Peninsula and the largest river in Myanmar. Its tributaries in China, the Longchuanjiang-Ruilijiang River, Dayingjiang River, Dulongjiang River and Mengnaihe River are rich in fish diversity, with a total of 84 fish species recorded. Due to insufficient sampling and diverse river habitats, there are still species that have not been discovered, described or documented from the basin. Several fish surveys conducted between 2021 and 2024 in Gaoligong Mountain (in the Irrawaddy River basin) of Baoshan City and Dehong Dai and Jingpo Autonomous Prefecture, Yunnan Province, found four newly recorded species of freshwater fish:Triplophysa nujiangensa, Homatula pycnolepis,Psilorhynchus olliei and Glyptothorax longinema (Fig. 1). [Methods] In this study, the collected specimens were identified and morphological characteristics were described with reference to research literatures of Triplophysa, Homatula, Psilorhynchus and Glyptothorax. Based on COI gene fragments, Bayesian inference tree was constructed by Phylosuite 1.2.2. All new sequences in this study have been uploaded to NCBI GenBank, and other sequences were obtained from NCBI GenBank database (Table 1). [Results] In this article, the four newly recorded species are described, morphological photos (Fig. 2, Figs. 4﹣7), morphological (Tables 2﹣5) data for identification and phylogenetic tree (Figs. 3, 8) are also provided. Based on morphological and molecular evidences, the specimens in this study are identified as T. nujiangensa, H. pycnolepis,P. olliei and G. longinema. [Conclusion] The first two species are the first records of two genera Triplophysa and Homatula in the Irrawaddy River basin. The third species represents an extended distribution of the Psilorhynchidaein China outside the Yarlung Zangbo River basin in Xizang. The last species, so far known from the Nujiang and Lancang Rivers, is the first record in IrrawaddyRiver basin. This study provides basic data for species identification of fishes in the Irrawaddy River basin of China, and a scientific basis for the investigation and conservation of fish resources in the Gaoligong Mountain area.