Abstract:Two small snakes were respectively collected in Shiwu Village (119°09′38″ E, 28°04′59″ N, altitude 225 m), Jianchi Streetand, and Lu’ao Village (119°12′25″ E, 27°55′25″ N, altitude 933 m), Lanju Town, Longquan, Lishui City, Zhejiang Province, China on October 8, 2021 and May 18, 2022. They are similar to Achalinus dehuaensis in morphology, with some slight differences in the type specimens. The snout-vent length and the tail length are longer than the type specimens. Twenty-one mitochondrial COI gene sequences of the genus Achalinus from GenBank were selected. Fimbrios klossi (Smith, 1921) and Parafimbrios lao (Teynié, David, Lottier, Le, Vidal & Nguyen, 2015) were chosen as outgroups. On the phylogenetic tree, the two newly collected samples clustered together with the holotype of A. dehuaensis (Voucher Number:YBU 13013) with high support (92%). The uncorrected pairwise distance between the two samples and the holotype of A. dehuaensis are 3.19%, less than those between the species of Achalinus (4.47%﹣23.34%). The newly collected specimens are identified to A. dehuaensis based on morphological and genetical data. It is a new record species of reptiles in Zhejiang Province. The specimens are deposited at the Anhui Normal University (Voucher number:ANU20230018 and ANU20230019).