The Eremias velox complex, a widespread racerunner in arid Central Asia, exhibits high morphological and genetic variability among different geographic populations. According to historical records, there are two subspecies of E. velox distributed in China, including E. v. velox from the Junggar Basin and the Ily River Valley, and E. v. roborowskii — elevated recently to a separate species E. roborowskii—endemic to the Turpan Basin. During herpetological surveys in May 2022 and July 2023, we captured three specimens of Eremias by hand near the Yiwanquan village of Gaochang District in Turpan City. To identify the taxonomic status of the three specimens, we used several methods as follow. We determined and complied the segments of the mitochondrial COIgene, and used Bayesian inference (BI) and maximum likelihood (ML) to reconstruct phylogenetic relationships. We calculated the uncorrected p-distance of different clades/subclades and compared the morphological characteristics.The morphological characteristics of the three specimens are consistent with those of E. v. velox (Fig. 2, 3 and Table 2), the results of phylogenetic relationships and haplotypes network (Fig. 4, 5) and genetic distance also indicated that the identity of the three specimens is E. v. velox.The occurrence of the nominate subspecies E. v. velox is a new reptile record of the Turpan Basin, which is also reported for the first time in the south of Tianshan Mountains. Our finding could provide basic data for further exploring the phylogeographic pattern and evolutionary history of the E. velox complex.