Peripheral blood cells were characterized in Polypedates megacephalus and Hylarana guentheri by the Wright-Giemsa staining method, and the data were compared with those previously recorded in Theloderma corticale. The results showed that the peripheral hematocytes of all three species were composed of erythrocytes, leukocytes and thrombocytes. The blood cells of the three species had the same commonality in morphology and structure (Fig. 1), while there were also remarkable differences in the shape, size and quantity (Fig. 2, Table 1﹣4). The nuclei of the red blood cells of H. guentheri were (23.56 ± 2.78) μm × (14.25 ± 1.62) μm in long diameter and short diameter, respectively, significantly larger than in P. megacephalus and T. corticale. There was no significant difference in size between neutrophils and lymphocytes, but the long diameter and short diameter of neutrophils and lymphocytes of T. corticale were the largest. In terms of the number of cells, the number of red blood cells in T. corticale was (24.33 ± 9.46) ×104/mm3, significantly lower than that in P. megacephalus or H. guentheri. while the number of white blood cells of P. megacephalus was (1.40 ± 0.40) ×104/mm3, which was the least among the three species, significantly different from the other two species (P < 0.05). These differences may be related to living environment and living habits of the frogs.