We observed washing behavior and courtship behavior of male adults of Chinese Giant Salamander Andrias davidianus in the imitated natural eco-breeding environment using all occurrence recording method at fixed time and fixed location and examined the significance of washing behavior for them by analyzing the relationship between the age, courtship behavior and breeding success. The washing behavior could be divided into four stages: washing exploration, gesture adjustment, washing and walk away with seven washing posture, head washing, tail washing, trunk washing, movement washing, surround washing, adherence washing and rising-head washing. Only males over 8 years showed washing behavior and courtship behavior after washing, and an extreme positive correlation between washing and courtship behaviors were detected. Two out of three male adults exhibited courtship behavior to participate in the breeding successfully. The washing behaviors maybe enhance gonadal development and maintain breeding successfully via courtship behavior.