To evalauate the safety of enrofloxacin for Carassius auratus gibelio, the acute toxicity of enrofloxacin and its influences on blood biochemical indicators were studied, based on the conventional dose of enrofloxacin in aquaculture. The results showed that the median lethal dose and safe dose of enrofloxacin for C.auratus gibelio were 1 949.84 mg/kg and 194.98 mg/kg, respectively. Compared with the control group, the content of serum total protein (TP), glutamic-pyruvic transaminase (ALT), aspartate aminotransferase (AST), and γ-glutamine transferase (γ-GT) were not significantly changed with the continuous oral administration at 20 mg/kg as a conventional dose for 30 days, while after the continuous oral administration at 40 mg/kg, 80 mg/kg, 160 mg/kg, 320 mg/kg for 30 days, the serum AST content was significantly increased by 23%, 30%, 46%, 86% (P<0.05), and the ALT content was significantly increased by 37%, 42%, 69%, 86% (P<0.05), respectively. This study determined the safe dose of enrofloxacin and its administration dose range causing liver dysfunction for C.auratus gibelio, and provided a scientific basis for the safe use of enrofloxacin in C.auratus gibelio. Enrofloxacin at 20 mg/kg as a conventional dose does not result in liver dysfunction in C.auratus gibelio.