Based on the literature review, questionnaires and trainer interview we designed a standard personality trait table to test and analyze the behavior of puppies 60-day-old. Three breeds, German Shepherd Dog, Labrador Retriever Dog, English Springer Spaniel were involved in this study. The results showed that the puppies showed their personality in three traits, i.e. sociability, playfulness, curiosity/fearlessness. The difference in personality between breeds was found. The playfulness showed by Labrador Retriever Dog was significantly higher than German Shepherd and Springer Spaniel (P<0.01); the sociability trait of German Shepherd was significantly higher than the Labrador Retriever and Springer Spaniel (P<0.01), and Labrador Retriever puppies displayed a significantly higher curiosity/fearlessness trait than German Shepherd and English Springer Spaniel (P<0.01). The results showed that the standard personality trait table has better reliability and validity, it can be used to describe and compare puppy individual difference.