Artificial propagation of Sturgeon has promoted the development of sturgeon aqualture in recent years. However, the genetic relationship among reserved broodstocks has not been analyzed. In order to avoid the degradation of germplasm resources and prevent inbreeding despression, the genetic background of reserved broodstocks should be investigated. In this research, partial sequence of mitochondrial control region (D-loop) was used to examine the genetic diversity of reserved broodstocks in four species of sturgeon. Different length of D-loop sequences between 451-469 bp were obtained from a total of 120 samples. There were 1-18 bp interspecific indels, while no intraspecific indels were detected. Each group contained at least four haplotypes which had greater than 98% sequence similarity. There were 4-12 pasimony informative sites between different haplotypes and genetic distance among intraspecific haplotypes varied from 0.002-0.024. The highest nucleotide diversity was presented in Sterlet (Acipenser ruthenus) (π=0.010) while the lowest was in Amur Sturgeon (A.schrenckii) (π=0.002). The highest haplotype diversity was showed in Siberian Sturgeon (A.baerii) (H=0.706) while the lowest was in Russian Sturgeon (A.gueldenstaedtii) (H=0.352). These results indicate that there is a low genetic diversity in the four reserved broodstocks and that inbreeding impact and genetic distance should be seriously considered in the fry production and germplasm conservation of the four species of sturgeon.