Pantholops hodgsonii is a unique wild animal species in China,and it is listed as an Appendix species by CITES and as Category Ⅰ by the Key Protected Wildlife List of China.Individuals of P.hodgsonii live in high altitude and low oxygen environment,with typical genetic characteristics of plateau animals.The P.hodgsoni fibroblast cells derived from a piece of ear margin tissue were successfully cultured and then purified by differential attachment in combination with digestion elimination methods.The morphology,dynamic growth,and karyotypes of the cultured cells at different passages were analyzed.The results showed that the cultured cells had typical characteristics of fibroblasts.The population doubling times (PDT) of passage 7,10 and 15 were 26 h,48 h,and 50 h,respectively.The growth curve of the cultured cells appeared "S" shape,and diploid cells (2n=60) were dominant (65%-91%).All of the chromosomes were telocentric (including X and Y chromosomes).The total and average relative length of chromosome set was 193.45 and 3.22, respectively.No satellites and secondary constriction were observed in the present study.