A wild population Tanichthys albonubes which has been listed a second-class state protected species was discovered in Guangxi, China. Compared with the wild population of Conghua, Guangdong, the fish found in Guangxi has obvious black strip on the dorsal fin, pectoral fin and anal fin, and its caudal peduncle is higher. The principal component analysis on the morphometric characters distinguished the population found in Guangxi from the population in Conghua, Guangdong. This fish inhabits in clear and acidic stream with fast current and lush float grass in the mountainous region.