The breeding behavior of Spot-billed Pelican (Pelecanus philippensis) was recorded by All-occurrence Recording Method in Shanghai Zoo from March 9^th to April 7^th, 2010. Three activity peaks occur in daylight hour at 6:00-7:00, 10:00-11:00 (male) or 11:00-12:00 (female), 14:00-15:00 respectively.The activity duration of males are significantly more than that of females (P<0.01).Males spend much more time to get nest materials than females (8.90%±5.04% vs. 6.19%±0.40%), while females spend more time to build nest than males (10.09%±4.81% vs.5.92%±2.62%) (P<0.01).Both male and female take turn in hatching eggs with an average exchange hatching frequency of 1.62±1.08 times/day, however, the female takes more time in hatching than the male (P<0.01).The results indicate that male and female cooperate closely in breeding activity.