Five bat specimens were collected from Nanling Nature Reserve in Guangdong Province in September 2009.The bat’s nostril is typical of the genus Murina,tubular and highly protuberant.The hairs in dorsal surface are dark brown basally,and the forearm length is 34.3-36.8 mm.Its karyotype is 2n=44 and FN=50.Based on the characteristics of external and skull morphologies,and karyotype,the specimens were identified as M.huttonii,which is new to Guangdong Province.The echolocation calls were FM signals.The karyotype and echolocation calls of M.huttonii Bat were firstly reported.