Choosing right statistical method is a key point to improve quality and credibility of scientific research.However,some methods used by biologists are often unreasonable or even incorrect.For instance,the normality and homogeneity of variance are often neglected in the t-test or Single-Factor Analysis of Variance.Two sample t tests are often employed to address the difference between multiple levels in one factor.The Pearson’s χ² has been frequently adopted in the contingency table,but inappropriate analyses and inferences are very common.Five examples from the wildlife literatures are used to illustrate the above questions.