The purpose of this study is to establish an efficient way for mouse parthenogenetic activation.Oocytes were recovered from mice at different hours after hCG administration and were denuded of cumulus cells with hyaluronidase.The cleavage and blastocyst rates of mouse oocytes activated with different concentrations of strontium chloride,and different durations of ethanol stimulation with or without 6-dimethylamino-purine,were analyzed.The cleavage rate of oocytes collected at 15-16,18-19,20-21 hours were increased after treatment with 6 mmol/L SrCl₂.The cleavage rate of oocytes collected at 20-21 hour was significantly higher than that of oocytes collected at 18-19 or 20-21 hour（P<0.05）.The blastocyst rate of oocytes collected at 18-19 hour was the highest among different groups.The cleavage rate of oocytes treated with 6 mmol/L and 10 mmol/L SrCl₂ were 76.4%,83.6%,and the morula rates were 50.0% and 56.3% respectively.The cleavage rate of oocytes treated with 70 ml/L ethanol for 7 min was 77.1%,and the blastocyst rate was also significantly higher than that of the other two groups（P<0.05）.The combination of 6-DMAP and SrCl₂ or ethanol,improved the extrusion of second polar body,and the diploid parthenogenetic embryonic rate.The average number of parthenogenetic blastocyst cells was significantly lower than that of normal blastocysts（P<0.05）.The cleavage of mouse parthenogenetic embryos depends on both the oocyte age and activation protocols.The parthenogenetic activation for oocytes collected at 18-19 hour by treatment with 10 mmol/L SrCl₂ or 70 ml/L ethanol combined with 6-DMAP,was the most efficient approach.