Abstract:Hynobius chinensis was re-discovered by Wang et al.from the type locality in 2005,116 years after its first description,which proves that H.chinensis is a true Hynobius species.In this paper,the karyotype of H.chinensis from the type locality was described by using embryos and staining with Giemsa.The results indicated that all individuals had a diploid number of 56 chromosomes and can be divided into four groups:the first group contained large biarmed m,sm,st chromosomes(Nos.1-9);Nos.10-13 composed the second,medium-sized group which contained m and sm chromosomes;the third group was composed of 5 pairs of metacentric chromosomes(Nos.14-18);the fourth group included 10(Nos.19-28)uniarmed microchromosomes.The results indicated that the karyotype of H.chinensis was similar to that of the other species with 2n=56 chromosomes.Although there were also some differences in the centromere type and grouping,the diploid chromosome number is not changed.