Two full-length cDNA sequences encoding C-reactive protein(CRP) gene of Chinese perch(Siniperca chuatsi) and α1-antitrypsin(AAT) gene of orange-spotted grouper(Epinephelus coioides) were isolated by RT-PCR and RACEs methods,respectively.The CRP cDNA is 914 bp in length,and contains a 666 bp open reading frame(ORF) encoding 222 amino acids.The AAT cDNA is 1 785 bp in length,and contains a 1 242 bp ORF encoding 414 amino acids.Sequence analysis reveals that the identity of CRP amino acids between amphibian and mammals is low,ranging at 22.4%-33.2%.The identity of AAT amino acid among fish,mammals and amphibian is also low,ranging at 36%-59.2%.The data are important for future study on molecular structure and function of disease-related genes in aquatic animals,and for improvement of the larvae survival and fry rearing during artificial propagation.