EYA4,homologous to eyes absent(eya)gene of Drosophila,is a member of the four vertebrate transcriptional activators of eya gene family.Previous studies have indicated that EYA4 gene is interrelated with sensorineural hearing loss.The specialized auditory system of bat that receives and processes echo information has an important role in echolocation.Using the RT-PCR method,the open reading frame(ORF)of EYA4 gene was cloned and sequenced in three species of bats(Myotis ricketti,Taphozous melanopogon,and Rousettus leschenaulti).Sequence alignment indicates that the EYA4 gene of all these three bats does not have exon 5 but has a unique exon b between exon 11 and exon 12.Compared to the EYA4 gene of other mammals,R.leschenaulti does not have exon 20 and T.melanopogon does not have exon 19.Moreover,T.melanopogon has a 30 bp deleted exon 16.M.ricketti has variable splicing of exon 19,exon 20 and exon a between exon 2 and exon 3.