The present paper deals with the toxic effects of Cu²⁺,Zn²⁺,Cd²⁺ in different ion concentration and the joint toxicity of Cu²⁺-Cd²⁺ and Pb²⁺-Zn²⁺ on the embryos development of Spinibarbus hollandi by using the semi-static test method,under the condition of water temperature between 24-26℃.The result suggests that the hatching rate of embryos incubated at the heavy metal solutions of different concentration is lower than the control,and the differences is extreme significant(P<0.01).Malformation of embryos was caused by the heavy metal element during the embryos development stage.The development speed of embryo was advanced in the experimental groups(Cu²⁺≥0.10 mg/L,Cu²⁺-Cd²⁺≥0.50 mg/L,Pb²⁺-Zn²⁺≥2.00 mg/L),but reduced in the rest of experimental groups of various concentration of metal solutions.The toxic effects of the heavy metals on the embryos development of S.hollandi are Cu²⁺>Cd²⁺>Zn²⁺.The safe concentration of Cu²⁺,Cd²⁺,Zn²⁺,Cu²⁺-Cd²⁺,Pb²⁺-Zn²⁺ are 0.009 mg/L,0.065 mg/L,0.023 mg/L,0.011 mg/L,0.523 mg/L respectively.The joint toxicity of Cu²⁺ and Cd²⁺ to the S.hollandi is antagonistic.