By means of switching mechanism at 5' end of RNA transcript(SMART)technology,single matured porcine MⅡ oocyte cDNAs were amplified.Meanwhile,the mRNA was amplified from the single porcine oocyte cDNA products using T7 RNA polymerase based in vitro transcription technology(IVT),and the aRNA concentration reached 1.962 μg.Smart technology combined with IVT technology,mRNA has augmented 2.3×10⁴ times,and this amount could meet the cDNA array and other high throughput screening requirements.Based on this research,a feasible platform has been established to carry out high throughput screening researches for precious and rare biological materials.