Immunohistochemical and morphometric methods were used in the present study to investigate the distribution and senescence-related changes of GFAP-immunoreactivity(IR)in the retina of lizard Gekko swinhonis.The results showed that the GFAP-IR was mainly localized in the vitread endfeet and inner part of the radial trunks of the Müller cells through tout the retina.Compared with adult lizards,the old lizards had a significant increase in the optical density(OD)and the percentage of GFAP-IR in the retinas.At the same time,the GFAP-IR Müller cells in the old lizards showed shorter minor diameters.All the results suggest that Müller cells probably participate in the senescencing processes of the retina by the mediation of the higher GFAP expression and lower decompositon.