The present paper deals with the urea lyses method for isolating spiders’ genomic DNAs.Compared to other two known methods,this method produced spider genomic DNAs with higher yields and higher integrity.It is also simpler and less time-consuming,involving only room temperature manipulations.High quality genomic DNAs were isolated from spider by this method.Certain spider dragline gene fragments,which contain highly repetitive sequences of high GC contents,were successfully PCR-amplified.