We conducted a bat species survey in Danxiashan National Nature Reserve by checking the daily/night roost sits and capturing bats by mist-net at their foraging area in the year 2017 and 2018. Totally, 23 species of 13 genera and 5 families, were found. Of 15 species (9 genera) were belonged to Vespertilionidae, 4 species (1 genus) were Rhinolophidae, 2 species (1 genus) were Hipposideridae, 1 species was Megadermatidae and 1 species was Molossidae (Table 1). For fauna composition of them 23, of 19 species belong to the Oriental kingdom, 1 species is Palaearctic kingdom and the rest 3 species are widely distributed in China. These bats have roosted at caves (15 species), arboreal (12 species) and buildings (5 species). Of them 9 species can roost at two kind of habitats. One species of bat Hypsugo cadornae was the first recorded in China. Myotis indochinensis has been confirmed distributed in China again.