Latitude variations in the life history characteristics of cavity-nesting birds, especially the breeding biology of tropical cavity-nesting birds, are still poorly understood. From March to August 2018, artificial nestboxes were set up in Diaoluoshan of Hainan, Dongzhai of Henan, and Saihanba of Hebei, China, to compare use of nestbox, hatching success and breeding success of cavity-nesting birds (Figure 1). A total of 577 nestboxes were set up in the field, among which the utilization rate was the lowest in Hainan (32.6%), while the highest was in Henan (92.0%) (Table 1). The Great Tit (Parus cinereus) was the most common cavity-nesting bird species found at three study sites, and there was no significant difference in hatching success and breeding success of Great Tits among three sites. However, hatching success (75.7%) and breeding success (65.7%) of Great Tits were significantly lower than those of Willow Tits (Poecile montanus) (97.7% and 97.7%) and Coal Tits (Periparus ater) (93.5% and 90.3%) within the same study area in Hebei (Table 2). Our study showed that there were variations with latitude in cavity-nesting bird species, species abundance and use of nestboxes. However, there were no significant differences in hatching success and breeding success for Great Tits, a common cavity-nesting bird across China.