Isolation and Purification of Sexual Progeny of Tetrahymena Thermophila

doi:10.1385.9/j.cjz.201902014

Home > Archive>Volume 54, Issue 2, 2019 >279-287. DOI:10.1385.9/j.cjz.201902014

Isolation and Purification of Sexual Progeny of Tetrahymena Thermophila
DOI:
                        10.1385.9/j.cjz.201902014
                    
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Affiliation:①Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072; ②College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing,100049,①Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072; ②College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing,100049,①Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072; ②College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing,100049,①Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072; ②College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing,100049,①Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072; ②College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing,100049,①Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072; ②College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing,100049
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Abstract:

When Tetrahymena cells are induced to mate for sexual reproduction in the lab, the resulting population always contains a certain proportion of unmated parental cells besides sexual progeny. However, highly purified sexual progeny is an important basis for studying some basic biological questions of Tetrahymena. In this study, based on the characteristics that mating Tetrahymena loses the ability to ingest particles and the newly produced sexual progeny does not recover this ability within several hours, we added the Fe3O4 magnetic nanoparticles to the mating population of T. thermophila at the late stage of sexual reproduction and only the unmated parent cells would become magnetic because they still had the ability to ingest the magnetic particles, which made possible an efficient isolation of sexual progeny under the action of magnetic field. By optimizing the time point at which cell population was passed through the separation column, the diameter of the column and the quality of iron/quartz powder in the column, we could make the purity and yield of the sexual progeny both more than 98%. The established method can also be used for the separation and purification of the sexual progeny of other Tetrahymena or ciliate species.

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MA Xue-Feng, 孙宗毅, 张晶, 陈凯, 王光营,MIAO Wei. 2019. Isolation and Purification of Sexual Progeny of Tetrahymena Thermophila. Chinese Journal of Zoology, 54(2): 279-287.

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Received:September 16,2018
Revised:February 14,2019
Adopted:February 12,2019
Online: March 25,2019
Published: April 20,2019

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