One specimen (♂) was found at Xianfei Cave (26°58′25″N, 113°3′23″E, altitude 463 m) of Sifang mountain, and nine more specimens (4♀ 5♂) were collected at Jinjue Mountain (26°58′24.4″N, 113°3′23.1″E, altitude 311 m) in August 2016 in Hengdong County, Hunan Province. The size of these specimens are small with a measurement of 33.3﹣36.1 mm in forearm length. The feet length (not including the length of the claws) exceeds half of the tibiae length, and the outer edge of the tibia is decorated with hair (Fig. 1). The greatest length of skull is 13.57﹣14.35 mm. The skull is thick and robust, the shape of neurocranium is round and obviously higher than the upper jaw bone (Fig. 2). These specimens were further confirmed as Myotis longipes by phylogenetic inferred from their Cyt b sequences (1 140 bp) (Table 1, Fig. 3). The divergence of these specimens was analyzed between the M. longipes of Guangxi, Laos, Chongqing and Guizhou from Hengdong (Table 3). Here, we provided the measurement data of the morphological structure, skull feature of the M. longipes, and compares them with the specimens in Guangdong Province, China and India (Table 2). The ten specimens were identified as M. longipes, which was first found in Hunan Province. The specimens have been kept in the Museum of Vertebrate Specimens at Hunan Normal University.