Charadriiformes (Vertebrate: Aves) is a species-rich order with approximately 384 species belonging to 94 genera of 19 families (Fig. 1), and contains some model species to study migration and foraging behavior. The resources of mitogenome have rapidly accumulated in recent years due to the advanced genomic sequencing, however, Charadriiformes mitogenome has not been well studied (Table 2). Primer design has crucial importance to PCR experiments, and a successful PCR experiment depends on the quality and specificity of the primers. After the multiple sequence alignment, we found many conserved regions. Then, we designed the forward and reverse primers according to the overlapping and conserved regions. In this study, we designed 13 pairs of primers, with the amplified target fragment for each pair about 1.5 kb in length (Fig. 2, Table 1). In order to test the proposed method and prove the performance of these primer pairs, the whole primer settings were applied to amplify and sequence the mitochondrial genomes of four birds: Grey-headed Lapwing Vanellus cinereus, Eurasian Woodcock Scolopax rusticola, Green Sandpiper Tringa ochropus, and Pin-tailed Snipe Gallinago stenura (Fig. 3). The results proved that these primer pairs were efficient. Taken together, the designed primer pairs are an effective toolkit of amplification and sequencing for the Charadriiformes mitogenomes. Our work provides a valuable resource facilitating further study of molecular systematics, population genetics, and phylogeography of Charadriiformes.