Plateau Pika (Ochotona curzoniac) is a small mammal native to Qinghai-Tibet area of 3 000﹣ 5 000 m above sea level. Previous studies found that the sperm-specific lactate dehydrogenase (LDH-C4) gene is expressed in Plateau Pika skeletal muscle. Intraperitoneal injection is a simple and convenient method for drug injection. In order to verify the effect of Ldhc silence in Plateau Pika skeletal muscle by intraperitoneal injection of adenovirus pMultiRNAi-Ldhc as a method of RNAi, 27 Pikas were randomly divided into the RNAi-LDHC, RNAi-HK and control groups, each had nine individuals. The Pikas of RNAi-LDHC, RNAi-HK were injected with 4 × 109 pfu 0.65 ml adenovirus pMultiRNAi-Ldhc and pMultiRNAi-NS, respectively. The control group was injected the same amount of normal saline. Seven days after injection, swimming time and the expression level of Ldhc mRNA and protein in Plateau Pika skeletal muscle, and the levels of lactate dehydrogenase and lactic acid as well as the content of ATP were detected. The results showed that, compared to the control group, RNAi-LDHC group′s swimming time reduced by 12.45% (Fig. 1). At the mRNA and protein levels, Ldhc gene expressions were reduced by 41.73% and 15.76%, respectively (Fig. 2). LDH, LD and ATP were reduced by 23.98%, 51.08% and 19.29%, respectively (Fig. 3). It is indicated that intraperitoneal injection of adenovirus pMultiRNAi-Ldhc can effectively silence Ldhc expression in Plateau Pika skeletal muscle.