The aim of this research is to explore the role of taurine in immunoregulation and oxidation defense in amphibians. The individuals of Hoplobatrachus rugulosus were administered with different dosages of taurine solution by gastric perfusion for 7 days. After that, respiratory burst of spleen macrophages, peripheral blood phagocytic activity and gastric lysozyme activity and concentrations of serum malonaldehyde (MDA) and glutathione (GSH) were determined. Statistical tests were performed using SPSS 17.0. Data are expressed as Mean ± Standard error of the mean (S.E.M.). To determine the existence of statistical differences between different concentrations, one-way ANOVA was used, followed by LSD test (with a degree of significance of P < 0.05). The results indicated that taurine increased both intensity of splenic macrophage respiratory burst and peripheral blood phagocytic activity in a dose-dependent manner, reaching to peak at 0.8 g/L. However, emergence of conditional toxicity was observed when the concentration of taurine exceeded 0.8 g/L (Fig. 1a and b). No significant change of gastric lysozyme activity was found among different taurine concentrations (Fig. 1c). Serum MDA content was decreased with increasing taurine concentrations, while serum GSH was increased in a dosage-dependent mannerin a certain concentration range (peak reached at 1 g/L) and the emergence of conditional toxicity was observed when the taurine concentration exceeded 1 g/L (Fig. 2a and b). Overall, taurine can markedly improve the immune function and increase antioxidant ability of the H. rugulosus within a certain concentration range and the best dosage to satisfy the nutritional requirement is about 16﹣20 mg/kg body weight basing on our investigations.