In this study, we conducted the comparative anatomy of the cloaca of the female Yaoshan Stout Newt, Pachytriton inexpectatus, and found that: the female Yaoshan Stout Newt cloaca possess numerous sperm storage related glands, spermathecae and ventral gland, in the roof of the cloaca. This suggests that the cloacae of salamanders have the morphological structures which are related to the function of sperm storage. According to the experimental results, it implies that the Yaoshan stout newt may mates and lays eggs more than once.