Stable isotope analysis samples require proper treatment before isotope mass spectrometry analysis, but there has not been established standard method of how to prepare the sample. Combining with the stable isotope analysis testing process of avian tissues, this paper introduces the sample pretreatment methods, such as cleaning, drying, isolation and purification, grinding, storage, and so on, which are involved in stable isotope technology applications of Ornithology; discusses the different tissues commonly used pretreatment methods and Sproblems to be solved in sample analysis. Aim to provide scientific reference and technical support for the application of stable isotope technique inSornithology