Lysozyme concentration in related to breeding density, laying order and Maternal Antibody of Newcastle Disease Virus in the Egg of Great Cormorant(Phalacrocorax Carbo)

doi:DOI: 10.13859/j.cjz.201602017

Home > Archive>Volume 51, Issue 2, 2016 >310-318. DOI:DOI: 10.13859/j.cjz.201602017

Lysozyme concentration in related to breeding density, laying order and Maternal Antibody of Newcastle Disease Virus in the Egg of Great Cormorant(Phalacrocorax Carbo)
DOI:
                        DOI: 10.13859/j.cjz.201602017
                    
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Affiliation:①Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810008, China;②Key Laboratory of Adaptation and Evolution of Plateau Biota, Chinese Academy of Science, Xining, 810008, China;③University of Chinese Academy of Science,,①Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810008, China;②Key Laboratory of Adaptation and Evolution of Plateau Biota, Chinese Academy of Science, Xining, 810008, China;③University of Chinese Academy of Science,,①Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810008, China;②Key Laboratory of Adaptation and Evolution of Plateau Biota, Chinese Academy of Science, Xining, 810008, China;③University of Chinese Academy of Science,,①Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810008, China;②Key Laboratory of Adaptation and Evolution of Plateau Biota, Chinese Academy of Science, Xining, 810008, China;③University of Chinese Academy of Science,,①Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810008, China;②Key Laboratory of Adaptation and Evolution of Plateau Biota, Chinese Academy of Science, Xining, 810008, China;③University of Chinese Academy of Science,,①Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810008, China;②Key Laboratory of Adaptation and Evolution of Plateau Biota, Chinese Academy of Science, Xining, 810008, China;③University of Chinese Academy of Science,,①Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810008, China;②Key Laboratory of Adaptation and Evolution of Plateau Biota, Chinese Academy of Science, Xining, 810008, China;③University of Chinese Academy of Science,,④Tibet plateau institute of biology，Lhasa,850001
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Abstract:

Female birds can transmit immune factors into eggs to defense pathogen by maternal effect, including innate immune factor lysozyme and adaptive maternal antibody. To investigate how the female birds allocate immune factors into eggs , we totally collected 20 clutches and 60 eggs of great cormorant (Phalacrocorax carbo) in Qinghai Lake and recorded the breeding density and laying order, then determining Newcastle Disease Virus antibody titer by Hemagglutinin Inhibition Test and lysozyme concentration by lysoplate assay, respectively. The Pearson correlation and Spearman correlation were employed to analyze the correlation between lysozyme concentration and breeding density and the correlation between NDV antibody titer and lysozyme concentration respectively, meanwhile, the relationship between lysozyme level and laying order was performed by One way ANOVA and student’s t test was applied to analyze the difference of lysozyme concentration in NDV positive eggs and negative eggs. From the results, we found that (1) lysozyme concentration was positively related to breeding density between clutches (r = 0.886, P < 0.0001, Pearson correlation, Table 1); (2) lysozyme concentration increased with laying order within a clutch (F = 3.95, P < 0.05, Fig 1) and the level of lysozyme in the NDV positive eggs was higher than in negative eggs (t = 4.08, df = 58, P<0.001, Fig 2); (3) the NDV antibody titer had negative relationship with lysozyme concentration in positive eggs (r =﹣0.840, P < 0.001, Table 2). Hence, we concluded that female birds transmit lysozyme into eggs according to breeding density and laying order, meanwhile, effectively transferring lysozyme and NDV maternal antibody into eggs to provide optimal protection for offspring.

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CAO Jian, WANG Wen, LI Ji-Rong, YANG Fang, WANG Xue-Lian, ZHENG Si-Si, LI Lai-Xing. 2016. Lysozyme concentration in related to breeding density, laying order and Maternal Antibody of Newcastle Disease Virus in the Egg of Great Cormorant(Phalacrocorax Carbo). Chinese Journal of Zoology, 51(2): 310-318.

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Received:July 28,2015
Revised:December 29,2015
Adopted:December 28,2015
Online: March 14,2016
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