During October 2012 and April 2013, we recorded the activities of the Asiatic Brush-tailed Porcupine, Atherurus macrourus in the subtropical upland evergreen broad-leaved forest (elevation at c. 1200m) of Yalu Tsangpo valley, Medog, Tibet, by using camera traps. Matching the occurrence of the Asiatic Brush-tailed Porcupine in each month and day with the moon period, we are aimed to discuss the correlation between the activity rhythm of the Asiatic Brush-tailed Porcupine and the phases of the moon. The results indicated that Asiatic Brush-tailed Porcupine was typically nocturnal with a single activity peak during 21:00 and 0:00. The activity rhythm of the Asiatic Brush-tailed Porcupine appeared to have a correlation with the phases of the moon (R2=0.8971): frequent occurrence were recorded at the new moon and waning crescent moon, while rare occurrence were at the full moon, and the occurrence during this period mostly concentrated on dawn and dusk; the occurrence were only recorded in in 20:00-1:00 at the waxing moon and 1:00-6:00 at the waning moon. The obvious avoidance of the Asiatic Brush-tailed Porcupine to the moonlight may bring about a low risk of predation, which is in accordance with the previous studies on other nocturnal rodents.