Creatine kinase（CK）plays an important role in the process of cell energy metabolism by catalyzing the reversible transfer of phosphate group between ADP and phosphocreatine. The cDNA full-length of the creatine kinase gene from Schizopygopisis pylzovi was cloned using RT-PCR and RACE methods, and then conducted the bioinformatics analyses. The results showed that the full-length cDNA of CK gene was 1599 bp, including a 1143 bp complete ORF encoding 380 amino acid peptide. The creatine kinase of Schizopygopsis pylzovi contains a N-terminal domain and a C-terminal catalysis domain. Sequence homologous analysis of amino acids showed that the CK of Schizopygopsis pylzovi has high homology with the M3-CK of Danio rerio and Cyprinus carpio, the identity reaches above 94%. Phylogenetic analysis showed that the CK of Schizopygopsis pylzovi and the M3-CK of Danio rerio and Cyprinus carpio were clustered together and formed a monophyletic group, from which the CK of Schizopygopsis pylzovi could be tentatively identified as the orthologous gene of the M3-CK of Danio rerio and Cyprinus carpio. The mRNA levels of major tissues of Schizopygopsis pylzovi were detected and analyzed using Quantitative Real-time PCR. The result showed that the CK transcript was highly expressed in muscle, intestine, eye and heart, while weakly expressed in hepatopancreas and brain. The high expression in muscle, intestine and heart of CK is consistent with its function in cell energy metabolism, but the high expression in eyes may be related to other functions of CK, need to further researches.